Journal: BMC Biotechnology
Article Title: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
doi: 10.1186/s12896-021-00695-6
Figure Lengend Snippet: Box-and-whisker plots of the DNA concentration (ng/μL) measured by ThermoScientific NanoDrop 2000™. 11 DNA extraction protocols were used, each with 6 individual adult, female T. circumcincta specimens. Boxes extend from the 25th to the 75th percentile of each group’s distribution of values; Whiskers above and below the box indicate the 10th and 90th percentiles. Median line __ ; Mean □ ; Outliers ♦; Dunn’s Multiple Comparison test significance between methods indicated by asterisk; *: 0.01 < p < 0.05; **: 0.001 < p < 0.01; ***: p < 0.001. Extraction method abbreviations: AccM (AccuPrep® Genomic DNA Extraction - Mammalian Tissue), AccW (AccuPrep® Genomic DNA Extraction Kit – Whole Blood, Buffy Coat and Cultured Cells), CheX (Chelex®100), CTAB (cetyl trimethyl ammonium bromide), EznF (E.Z.N.A.® Forensic DNA), IsoG (Isolate II Genomic DNA Kit), Schi ( Schistosoma sp. DNA Extraction Method), Schi-LE (Schi with larval exsheathment), SDS (sodium dodecyl sulphate), WizM (Wizard® Genomic DNA Purification Kit - Mouse Tail), WizP (Wizard® Genomic DNA Purification - Plant Tissue)
Article Snippet: Wizard® Genomic DNA Purification Kit - Mouse Tail , WizM , Promega (A1120) , Precipitation , 4–4.5 a.
Techniques: Whisker Assay, Concentration Assay, DNA Extraction, Comparison, Extraction, Cell Culture, DNA Purification